A bioluminescent earthworm luciferase mimetic MIL-101(Cr)-MOF for enhanced luciferin chemiluminescence and H2O2 sensing

• Published in: Journal of photochemistry and photobiology. A, Chemistry. Chemistry., 2023-03, Vol.437, p.114332, Article 114332
• Main Authors: Nemati, Afsaneh, Chaichi, Mohammad Javad, Lakouraj, Moslem Mansour, Hosseinkhani, Saman, Seyedalipour, Bagher
• Format: Article
• Language: English
• Subjects: Box–Behnken design; Luciferase mimetic; Luciferin; Luminol; MIL-101(Cr)
• ISSN: 1010-6030; 1873-2666
• DOI: 10.1016/j.jphotochem.2022.114332

[Display omitted] •Luminol embedded into MIL-101 (Cr) -MOF was synthesized as a luciferase mimetic.•Luciferase mimetic was explored in earthworm bioluminescence system for sensing H2O2.•Luciferase mimetic has a catalytic role for the luciferin CL system in H2O2 sensing.•Response surface methodology based on BBD was applied to optimize level of factors. A sensitive and rapid method was introduced in this research for the detection of H2O2 based on luciferase mimetic. Luciferase mimetic of bioluminescent earthworm was synthesized using a metal–organic framework (MOF). The luminol was loaded into the chromium terephthalate MOF MIL-101(Cr) pores and applied in the luciferin chemiluminescence (CL) system with an outstanding performance. The samples exhibited prominent catalytic performance of luciferase mimetic in the luciferin chemiluminescence system for H2O2 sensing with high sensitivity in the linear range of 1 × 10−2– × 10−10 mol/L and detection limit of 1.251 × 10−11 mol/L (R2 = 0.9944). The effects of the key factors were explored by response surface methodology (RSM) based on a Box–Behnken design (BBD). Thanks to its features such as low cost, rapid response, and portability, this enzyme mimetic could be a promising platform for CL sensing, offering great potential for the construction of a CL H2O2 sensor for biotechnology and clinical diagnostic purposes.