Secretome analysis of atherosclerotic and non-atherosclerotic arteries reveals dynamic extracellular remodeling during pathogenesis

• Published in: Journal of proteomics 2012-06, Vol.75 (10), p.2960-2971
• Main Authors: de la Cuesta, Fernando, Barderas, Maria G., Calvo, Enrique, Zubiri, Irene, Maroto, Aroa S., Darde, Veronica M., Martin-Rojas, Tatiana, Gil-Dones, Felix, Posada-Ayala, Maria, Tejerina, Teresa, Lopez, Juan A., Vivanco, Fernando, Alvarez-Llamas, Gloria
• Format: Article
• Language: English
• Subjects: Atheroma plaque; atherosclerosis; Atherosclerosis (general aspects, experimental research); Biological and medical sciences; Blood and lymphatic vessels; Cardiology. Vascular system; Coronary artery; coronary vessels; Diverse techniques; Fundamental and applied biological sciences. Psychology; humans; immunohistochemistry; Medical sciences; Molecular and cellular biology; pathogenesis; phosphoglucomutase; proteins; proteomics; Secretome; Vascular disease
• ISSN: 1874-3919
• DOI: 10.1016/j.jprot.2011.12.005

Early detection of cardiovascular diseases and knowledge of underlying mechanisms is essential. Tissue secretome studies resemble more closely to the in vivo situation, showing a much narrower protein concentrations dynamic range than plasma. This study was aimed to the analysis of human arterial tissue secretome and to the quantitative comparison of healthy and atherosclerotic secretome to discover proteins with key roles in atherosclerosis development. Secretomes from three biological replicates of human atherosclerotic coronary arteries (APC), preatherosclerotic coronaries (PC) and mammaries (M) were analyzed by LC-MS/MS. The identified proteins were submitted to Ingenuity Pathway Analysis (IPA) tool. Label-free MS/MS based quantification was performed and validated by immunohistochemistry. 64 proteins were identified in the 3 replicates of at least one of the 3 groups and 15 secreted proteins have not been previously reported in plasma. Four proteins were significantly released in higher amounts by mammary tissue: gelsolin, vinculin, lamin A/C and phosphoglucomutase 5. The study of tissue secretome reveals key proteins involved in atherosclerosis which have not been previously reported in plasma. Novel proteins are here highlighted which could be potential therapeutic targets in clinical practice. This article is part of a Special Issue entitled: Proteomics: The clinical link. [Display omitted] ► Quantitative analysis of atherosclerotic human arterial tissue secretome is here performed. ► Gelsolin, vinculin, lamin A/C and phosphoglucomutase 5 are highly released by healthy tissue. ► Secretome reveals key proteins in atherosclerosis, not previously reported in plasma.