Proteomic analysis identifies a novel function for galectin-3 in the cell entry of parvovirus

Cellular factors associated with the parvovirus minute virus of mice (MVM) during infection are thought to play important roles in the MVM life cycle but only a few of these have been identified. Here we used a proteomic-based approach in order to identify host-binding partners of MVM. Using purifie...

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Bibliographic Details
Published in:Journal of proteomics 2013-02, Vol.79, p.123-132
Main Authors: Garcin, Pierre, Cohen, Sarah, Terpstra, Sanne, Kelly, Isabelle, Foster, Leonard J., Panté, Nelly
Format: Article
Language:English
Subjects:
endocytosis
fibroblasts
Galectin-3
mass spectrometry
mice
Minute virus of mice
Parvovirus
pathogenicity
plasma membrane
precipitin tests
proteins
Proteomics
small interfering RNA
Viral cell entry
Virus
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Summary:Cellular factors associated with the parvovirus minute virus of mice (MVM) during infection are thought to play important roles in the MVM life cycle but only a few of these have been identified. Here we used a proteomic-based approach in order to identify host-binding partners of MVM. Using purified MVM as bait for immunoprecipitation assays, a total of 150 proteins were identified in MVM immunoprecipitates by quantitative liquid chromatography–tandem mass spectrometry. Galectin-3 was one of six proteins showing a statistically significant enrichment across replicates. Small interfering RNA depletion studies revealed an important role for galectin-3 in MVM endocytosis and infectivity in LA9 mouse fibroblast cells. Galectin-3-depleted cells were less susceptible to MVM infection than control cells and showed a significant reduction of MVM cellular uptake, but not of MVM binding to the cell surface. Our results indicate an important role for galectin-3 in the cellular uptake of MVM. We propose that galectin-3 facilitates the access of MVM to its receptor(s) at the plasma membrane and in this way promotes MVM endocytosis. [Display omitted] ► Proteomic approach identified host-binding partners of the parvovirus minute virus of mice (MVM). ► A total of 150 proteins were identified. ► Galectin-3 was one of six proteins showing a statistically significant enrichment across replicates. ► Role of galectin-3 was studied in cells infected with MVM. ► siRNA studies revealed an important role for galectin-3 in MVM endocytosis and infectivity.
ISSN:1874-3919
DOI:10.1016/j.jprot.2012.12.010