Analysis of the regulation of the Ustilago maydis proteome by dimorphism, pH or MAPK and GCN5 genes

• Published in: Journal of proteomics 2013-02, Vol.79, p.251-262
• Main Authors: Martínez-Salgado, José L., León-Ramírez, Claudia G., Pacheco, Alberto Barrera, Ruiz-Herrera, José, de la Rosa, Ana P. Barba
• Format: Article
• Language: English
• Subjects: Constitutive mutants; corn; Dimorphism; genes; haploidy; mitogen-activated protein kinase; mutation; mycelium; proteins; proteome; Proteomics; reactive oxygen species; toxicity; transcription factors; Two-dimensional gel electrophoresis; Ustilago maydis; Ustilago zeae; yeasts
• ISSN: 1874-3919
• DOI: 10.1016/j.jprot.2012.12.022

Ustilago maydis is a dimorphic corn pathogenic basidiomycota whose haploid cells grow in yeast form at pH7, while at pH3 they grow in the mycelial form. Two-dimensional gel electrophoresis (2-DE) coupled with LC-ESI/MS-MS was used to analyze the differential accumulation of proteins in yeast against mycelial morphologies. 2-DE maps were obtained in the pH range of 5–8 and 404 total protein spots were separated. From these, 43 were differentially accumulated when comparing strains FB2wt, constitutive yeast CL211, and constitutive mycelial GP25 growing at pH7 against pH3. Differentially accumulated proteins in response to pH are related with defense against reactive oxygen species or toxic compounds. Up-accumulation of CipC and down-accumulation of Hmp1 were specifically related with mycelial growth. Changes in proteins that were affected by mutation in the gene encoding the adaptor of a MAPK pathway (CL211 strain) were UM521* and transcription factors Btf3, Sol1 and Sti1. Mutation of GCN5 (GP25 strain) affected the accumulation of Rps19-ribosomal protein, Mge1-heath shock protein, and Lpd1-dihydrolipoamide dehydrogenase. Our results complement the information about the genes and proteins related with the dimorphic transition in U. maydis and changes in proteins affected by mutations in a MAPK pathway and GCN5 gene. [Display omitted] ► Ustilago maydis proteome map was most representative in the pH range of 5–8. ► Ustilago maydis can form filaments in vitro in response to acidic pH. ► Constitutive yeast and mycelia mutants (CL211 and GP25, respectively) ► Dimorphic related proteins and mutants related proteins were detected.