Comparative proteomics of uropathogenic Escherichia coli during growth in human urine identify UCA-like (UCL) fimbriae as an adherence factor involved in biofilm formation and binding to uroepithelial cells

Uropathogenic Escherichia coli (UPEC) are the primary cause of urinary tract infection (UTI) in humans. For the successful colonisation of the human urinary tract, UPEC employ a diverse collection of secreted or surface-exposed virulence factors including toxins, iron acquisition systems and adhesin...

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Bibliographic Details
Published in:Journal of proteomics 2016-01, Vol.131, p.177-189
Main Authors: Wurpel, Daniël J., Totsika, Makrina, Allsopp, Luke P., Webb, Richard I., Moriel, Danilo G., Schembri, Mark A.
Format: Article
Language:English
Subjects:
Adhesion
Chaperone-usher
F17-like
Outer membrane
Pili
Proteome
UPEC
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Summary:Uropathogenic Escherichia coli (UPEC) are the primary cause of urinary tract infection (UTI) in humans. For the successful colonisation of the human urinary tract, UPEC employ a diverse collection of secreted or surface-exposed virulence factors including toxins, iron acquisition systems and adhesins. In this study, a comparative proteomic approach was utilised to define the UPEC pan and core surface proteome following growth in pooled human urine. Identified proteins were investigated for subcellular origin, prevalence and homology to characterised virulence factors. Fourteen core surface proteins were identified, as well as eleven iron uptake receptor proteins and four distinct fimbrial types, including type 1, P, F1C/S and a previously uncharacterised fimbrial type, designated UCA-like (UCL) fimbriae in this study. These pathogenicity island (PAI)-associated fimbriae are related to UCA fimbriae of Proteus mirabilis, associated with UPEC and exclusively found in members of the E. coli B2 and D phylogroup. We further demonstrated that UCL fimbriae promote significant biofilm formation on abiotic surfaces and mediate specific attachment to exfoliated human uroepithelial cells. Combined, this study has defined the surface proteomic profiles and core surface proteome of UPEC during growth in human urine and identified a new type of fimbriae that may contribute to UTI. [Display omitted] •Analysis of the UPEC OM-associated proteome following growth in human urine•Definition of a core UPEC surface proteome associated with growth in human urine•Detection of UCA-like fimbrial proteins from UPEC•Functional characterisation of UCA-like fimbriae
ISSN:1874-3919
DOI:10.1016/j.jprot.2015.11.001