Peptidomics of Acanthoscurria gomesiana spider venom reveals new toxins with potential antimicrobial activity

Acanthoscurria gomesiana is a Brazilian spider from the Theraphosidae family inhabiting regions of Southeastern Brazil. Potent antimicrobial peptides as gomesin and acanthoscurrin have been discovered from the spider hemolymph in previous works. Spider venoms are also recognized as sources of biolog...

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Bibliographic Details
Published in:Journal of proteomics 2017-01, Vol.151, p.232-242
Main Authors: Abreu, Thiago F., Sumitomo, Bianca N., Nishiyama, Milton Y., Oliveira, Ursula C., Souza, Gustavo H.M.F., Kitano, Eduardo S., Zelanis, André, Serrano, Solange M.T., Junqueira‐de‐Azevedo, Inácio, Silva, Pedro I., Tashima, Alexandre K.
Format: Article
Language:English
Subjects:
Acanthoscurria gomesiana
Antimicrobial peptide
Peptidomics
Theraphotoxin
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Summary:Acanthoscurria gomesiana is a Brazilian spider from the Theraphosidae family inhabiting regions of Southeastern Brazil. Potent antimicrobial peptides as gomesin and acanthoscurrin have been discovered from the spider hemolymph in previous works. Spider venoms are also recognized as sources of biologically active peptides, however the venom peptidome of A. gomesiana remained unexplored to date. In this work, a MS-based workflow was applied to the investigation of the spider venom peptidome. Data-independent and data-dependent LC-MS/MS acquisitions of intact peptides and of peptides submitted to multiple enzyme digestions, followed by automated chromatographic alignment, de novo analysis, database and homology searches with manual validations showed that the venom is composed by
ISSN:1874-3919
DOI:10.1016/j.jprot.2016.07.012