Peroxiredoxin-2 and zinc-alpha-2-glycoprotein as potentially combined novel salivary biomarkers for early detection of oral squamous cell carcinoma using proteomic approaches

No effective screening method is available for oral squamous cell carcinoma (OSCC) that is recognized to influence by environmental factors as well as human papillomavirus (HPV) and Epstein-Barr virus (EBV). Therefore, we sought to identify salivary biomarkers for screening of OSCC with or without H...

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Bibliographic Details
Published in:Journal of proteomics 2018-02, Vol.173, p.52-61
Main Authors: Heawchaiyaphum, Chukkris, Pientong, Chamsai, Phusingha, Pensiri, Vatanasapt, Patravoot, Promthet, Supannee, Daduang, Jureerut, Teeramatwanich, Watchareporn, Kongyingyoes, Bunkerd, Chuerduangphui, Jureeporn, Ekalaksananan, Tipaya
Format: Article
Language:English
Subjects:
Epstein-Barr virus
Human papillomavirus
Oral squamous cell carcinoma
Peroxiredoxin-2
Saliva
Zinc-alpha-2-glycoprotein
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Summary:No effective screening method is available for oral squamous cell carcinoma (OSCC) that is recognized to influence by environmental factors as well as human papillomavirus (HPV) and Epstein-Barr virus (EBV). Therefore, we sought to identify salivary biomarkers for screening of OSCC with or without HPV and/or EBV infection. Saliva, lesion and oral exfoliated cells were collected from OSCC patients and cancer-free controls (CFCs) and grouped depending on their HPV- and EBV-infection status. Salivary protein was precipitated and subjected to 2-dimensional gel electrophoresis. Differential expression of proteins was identified by mass spectrometry and validated by Western blotting. Distinctive expression patterns of salivary proteins were detected in OSCC as compared with CFCs. Levels of peroxiredoxin-2 (PRDX-2) and zinc-alpha-2-glycoprotein (ZAG) were significantly up-regulated in OSCC cases (p
ISSN:1874-3919
DOI:10.1016/j.jprot.2017.11.022