Assessment of candidate biomarkers in paired saliva and plasma samples from oral cancer patients by targeted mass spectrometry

For oral cancer, numerous saliva- and plasma-derived protein biomarker candidates have been discovered and/or verified; however, it is unclear about the behavior of these candidates as saliva or plasma biomarkers. In this study, we developed two targeted assays, MRM and SISCAPA-MRM, to quantify 30 p...

Full description

Saved in:
Bibliographic Details
Published in:Journal of proteomics 2020-01, Vol.211, p.103571, Article 103571
Main Authors: Chi, Lang-Ming, Hsiao, Yung-Chin, Chien, Kun-Yi, Chen, Szu-Fan, Chuang, Yao-Ning, Lin, Shih-Yu, Wang, Wei-Shun, Chang, Ian Yi-Feng, Yang, Chi, Chu, Lichieh Julie, Chiang, Wei-Fan, Chien, Chih-Yen, Chang, Yu-Sun, Chang, Kai-Ping, Yu, Jau-Song
Format: Article
Language:English
Subjects:
Biomarker
MRM-MS
Oral cancer
Plasma
Quantification
Saliva
SISCAPA-MRM
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:For oral cancer, numerous saliva- and plasma-derived protein biomarker candidates have been discovered and/or verified; however, it is unclear about the behavior of these candidates as saliva or plasma biomarkers. In this study, we developed two targeted assays, MRM and SISCAPA-MRM, to quantify 30 potential biomarkers in both plasma and saliva samples collected from 30 healthy controls and 30 oral cancer patients. Single point measurements were used for target quantification while response curves for assay metric determination. In comparison with MRM assay, SISCAPA-MRM effectively improved (>1.5 fold) the detection sensitivity of 11 and 21 targets in measurement of saliva and plasma samples, respectively. The integrated results revealed that the salivary levels of these 30 selected biomarkers weakly correlated (r 
ISSN:1874-3919
DOI:10.1016/j.jprot.2019.103571