TMT-labelled quantitative proteomic analysis to identify the proteins underlying radiation-induced colorectal fibrosis in rats

Radiation-induced colorectal fibrosis (RICF) after pelvic radiotherapy can lead to stenosis and affect patients' quality of life. However, to date, few effective prevention and treatment methods for RICF have been implemented in clinical practice. Therefore, there is a strong need to investigat...

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Bibliographic Details
Published in:Journal of proteomics 2020-07, Vol.223, p.103801, Article 103801
Main Authors: Huang, Shenghui, Wang, Xiaojie, Sun, Yanwu, Lu, Xingrong, Jiang, Weizhong, Chen, Zhifen, Huang, Ying, Chi, Pan
Format: Article
Language:English
Subjects:
Intestinal fibrosis
Proteomics
Radiation proctitis
Radiation-induced fibrosis
Radiotherapy
Radiotoxicity
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Summary:Radiation-induced colorectal fibrosis (RICF) after pelvic radiotherapy can lead to stenosis and affect patients' quality of life. However, to date, few effective prevention and treatment methods for RICF have been implemented in clinical practice. Therefore, there is a strong need to investigate the molecular events underlying radiation-induced intestinal fibrosis. This study aimed to identify the comprehensive protein expression profiles related to RICF in rats. We performed tandem mass tag (TMT)-labelling proteomic analysis to examine RICF in rats after a single dose of 20 Gy conformal irradiation via X-ray and confirmed the altered proteins with parallel reaction monitoring (PRM). In total, we identified 6692 proteins, of which 5756 were quantified. When the p-value was 1.5 or 
ISSN:1874-3919
DOI:10.1016/j.jprot.2020.103801