TMT proteomics analysis of intestinal tissue from patients of irritable bowel syndrome with diarrhea: Implications for multiple nutrient ingestion abnormality

Diarrheal irritable bowel syndrome (IBS-D) is a chronic functional bowel disease with no clear diagnostic markers and no satisfactory treatment strategies. In recent years, the importance of intestinal microstructure and function in IBS-D has been emphasized. However, the intestinal tissue proteomic...

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Bibliographic Details
Published in:Journal of proteomics 2021-01, Vol.231, p.103995, Article 103995
Main Authors: Chai, Yu-na, Qin, Jin, Li, Yan-le, Tong, Ya-lin, Liu, Guang-hui, Wang, Xin-ru, Liu, Cheng-ye, Peng, Ming-hang, Qin, Chong-zhen, Xing, Yu-rong
Format: Article
Language:English
Subjects:
Bioinformatics analysis
IBS-D
Nutrient ingestion
Parallel reaction monitoring
Proteomics
Tandem mass tag
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Summary:Diarrheal irritable bowel syndrome (IBS-D) is a chronic functional bowel disease with no clear diagnostic markers and no satisfactory treatment strategies. In recent years, the importance of intestinal microstructure and function in IBS-D has been emphasized. However, the intestinal tissue proteomics of IBS-D patients has not been analyzed. Here, we systematically analyzed the molecule profiling of the intestinal tissues in IBS-D patients through tandem mass tag (TMT)-based proteomics for the first time, aiming to reveal the pathogenesis and provide evidence for diagnosis and treatment of IBS-D. Five IBS-D patients and five healthy subjects were selected, biopsy tissue samples from the junction of sigmoid and rectum were analyzed by TMT proteomics. Differentially expressed proteins were obtained and bioinformatics analysis was performed. Furthermore, parallel reaction monitoring (PRM) and q-PCR detection were applied to validate the differentially expressed proteins. Eighty differentially expressed proteins were screened, 48 of which were up-regulated and 32 were down-regulated (fold change >1.2, P 
ISSN:1874-3919
DOI:10.1016/j.jprot.2020.103995