Quantitative proteomics of cerebrospinal fluid using tandem mass tags in dogs with recurrent epileptic seizures

This prospective study included four dog groups (group A: healthy dogs, groups B: dogs with idiopathic epilepsy under antiepileptic medication (AEM), C: idiopathic epilepsy dogs without AEM administration, D: dogs with structural epilepsy). The purpose of the study was to compare the proteomic profi...

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Bibliographic Details
Published in:Journal of proteomics 2021-01, Vol.231, p.103997, Article 103997
Main Authors: Baka, Rania, Eckersall, David, Horvatic, Anita, Gelemanovic, Andrea, Mrljak, Vladimir, McLaughlin, Mark, Athanasiou, Labrini V., Papaioannou, Nikolaos, Stylianaki, Ioanna, Hanh, Han Quang, Chadwick, Christopher C., Polizopoulou, Zoe
Format: Article
Language:English
Subjects:
Dogs
Idiopathic epilepsy
SPARCL immunoassay
TMT-based proteomics
Western blot
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Summary:This prospective study included four dog groups (group A: healthy dogs, groups B: dogs with idiopathic epilepsy under antiepileptic medication (AEM), C: idiopathic epilepsy dogs without AEM administration, D: dogs with structural epilepsy). The purpose of the study was to compare the proteomic profile among the four groups. Samples were analyzed by a quantitative Tandem Mass Tags approach using a Q-Exactive-Plus mass-spectrometer. Identification and relative quantification were performed using Proteome Discoverer, and data were analyzed using R. Gene ontology terms were analyzed based on Canis lupus familiaris database. Data are available via ProteomeXchange with identifier PXD018893. Eighteen proteins were statistically significant among the four groups (P 
ISSN:1874-3919
DOI:10.1016/j.jprot.2020.103997