Quantitative serum proteome analysis using tandem mass tags in dogs with epilepsy

This study included four groups of dogs (group A: healthy controls, group B: idiopathic epilepsy receiving antiepileptic medication (AEM), group C: idiopathic epilepsy without AEM, group D: structural epilepsy). Comparative quantitative proteomic analysis of serum samples among the groups was the ma...

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Bibliographic Details
Published in:Journal of proteomics 2024-01, Vol.290, p.105034, Article 105034
Main Authors: Baka, Rania D., Kuleš, Josipa, Beletić, Anđelo, Farkaš, Vladimir, RešetarMaslov, Dina, Ljubić, Blanka Beer, Rubić, Ivana, Mrljak, Vladimir, McLaughlin, Marκ, Eckersall, David, Polizopoulou, Zoe
Format: Article
Language:English
Subjects:
Dog
Haptoglobin binding assay
Idiopathic epilepsy
Serum
Specific ELISA assay
TMT-based proteomics
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Summary:This study included four groups of dogs (group A: healthy controls, group B: idiopathic epilepsy receiving antiepileptic medication (AEM), group C: idiopathic epilepsy without AEM, group D: structural epilepsy). Comparative quantitative proteomic analysis of serum samples among the groups was the main target of the study. Samples were analyzed by a quantitative Tandem-Mass-Tags approach on the Q-Exactive-Plus Hybrid Quadrupole-Orbitrap mass-spectrometer. Identification and relative quantification were performed in Proteome Discoverer. Data were analyzed using R. Gene ontology terms were analyzed based on Canis lupus familiaris database. Data are available via ProteomeXchange with identifier PXD041129. Eighty-one proteins with different relative adundance were identified in the four groups and 25 were master proteins (p 
ISSN:1874-3919
DOI:10.1016/j.jprot.2023.105034