Proliferation of seeded urothelial and smooth muscle cells on different collagen scaffolds and commercially available small intestinal submucosa

• Published in: Journal of pediatric urology 2007, Vol.3, p.S57-S57
• Main Author: Luc ROELOFS, Jody NUININGA, Herman VAN MOERKERK , Willeke DAAMEN , Egbert OOSTERWIJK, Toin VAN KUPPEVELT and Wout FEITZ
• Format: Article
• Language: English
• Subjects: Pediatrics; Urology
• ISSN: 1477-5131; 1873-4898
• DOI: 10.1016/j.jpurol.2007.01.095

PURPOSE Different scaffolds have been used for bladder wall regeneration. In search for better materials we have developed collagen scaffolds and compared the in-vitro viability and proliferation of seeded urothelial and smooth muscle cells on 3 different collagen scaffolds and the commercially available small intestinal submucosa (SIS® ). MATERIAL AND METHODS The collagen scaffolds were created of bovine collagen type I, and chemically cross-linked: 1) porous scaffold; 2) dual-layer scaffold, combining a film-layer with a porous layer; 3) porous scaffold with one side closed. Urothelial cells (SCaBER cell line) and smooth muscle cells (PM151T cell line) were separately seeded on the collagen and SIS® scaffolds, and cultured under standard conditions. After 3, 7, 14 and 21 days cell viability and proliferation was analysed with the WST-1 assay. Univariate ANOVA test was used for statistical analysis. Seeded scaffolds were paraffin embedded and analyzed by H&E, cytokeratins AE1/AE3, E-cadherin, α-smooth muscle actin and desmin staining. RESULTS Progressive growth of seeded cells was observed on all scaffolds. Collagen scaffolds showed better proliferation and viability of cells in comparison to SIS® (p