Effects of spinosad on the heat tolerance and cold tolerance of Sitophilus oryzae L. (Coleoptera: Curculionidae) and Rhyzopertha dominica F. (Coleoptera: Bostrichidae)

• Published in: Journal of stored products research 2018-06, Vol.77, p.84-88
• Main Authors: Wijayaratne, L.K.W., Rajapakse, R.H.S.
• Format: Article
• Language: English
• Subjects: Cold tolerance; Heat tolerance; Reduced-risk insecticides; Stored-product insects; Synergy
• ISSN: 0022-474X; 1879-1212
• DOI: 10.1016/j.jspr.2018.03.001

Sitophilus oryzae and Rhyzopertha dominica are serious insect pests of stored products in Sri Lanka. Currently pirimiphos methyl and phosphine fumigation are used as control measures but grain handlers seek for alternatives. Exposure to high or low temperature is popular in stored-product insect pest management but is expensive. Spinosad is effective against certain stored-product insects but has not yet been tested for its synergy with heat or cold. This experiment was conducted to evaluate the effect of spinosad on the heat tolerance and cold tolerance of S. oryzae and R. dominica adults. The experiment was a two-factor factorial, complete randomized design with four replicates. The spinosad concentration and exposure period were changed. Adults of S. oryzae and R. dominica were first exposed to a series of spinosad concentrations. Later they were held at higher (40 °C) or lower (6–11 °C) temperatures than room temperature for different durations. Pre exposure of S. oryzae adults to Spinosad at 18 ppm or above synergized the adult mortality at high or low temperature showing a dose response. Pre-exposure of R. dominica adults to spinosad concentrations 12.5 ppm or higher synergized the mortality at high temperature whereas the spinosad synergized the mortality of R. dominica at low temperature when exposed to 6.25 ppm or higher concentrations; the effects followed a dose response. This study shows that heat and cold tolerance of S. oryzae and R. dominica adults are reduced by pre-exposure to spinosad. Therefore, spinosad is a potential grain protectant at high or low temperatures against these two insect species. •Spinosad was tested for effect on heat tolerance and cold tolerance of Sitophilus oryzae and Rhyzopertha dominica.•Pre-exposure of S. oryzae and R. dominica adults to spinosad at high concentrations reduced the heat and cold tolerance.•Effects of spinosad on the heat tolerance and cold tolerance of S. oryzae and R. dominica followed a dose response.