Induction of HL-60 apoptosis by ethyl acetate extract of Cordyceps sinensis fungal mycelium

The cultivated mycelium of a Cordyceps sinensis (Cs) fungus was sequentially extracted by petroleum ether, ethyl acetate (EtOAc), ethanol and water. The EtOAc extract showed the most potent cytotoxic effect against the proliferation of human premyelocytic leukemia cell HL-60, with an ED 50 ≤ 25 μg/m...

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Bibliographic Details
Published in:Life sciences (1973) 2004-10, Vol.75 (24), p.2911-2919
Main Authors: Zhang, Qiaoxia, Wu, Jianyong, Hu, Zongding, Li, Duan
Format: Article
Language:English
Subjects:
Acetates - chemistry
Apoptosis
Apoptosis - drug effects
Caspase 3
Caspases - metabolism
Chromatin - drug effects
Cordyceps - chemistry
Cordyceps sinensis
Cytotoxicity
DNA Fragmentation - drug effects
Enzyme Activation - drug effects
HL-60
HL-60 Cells
Humans
Mycelium - chemistry
Mycelium extract
Poly(ADP-ribose) Polymerases - metabolism
Tissue Extracts - toxicity
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Summary:The cultivated mycelium of a Cordyceps sinensis (Cs) fungus was sequentially extracted by petroleum ether, ethyl acetate (EtOAc), ethanol and water. The EtOAc extract showed the most potent cytotoxic effect against the proliferation of human premyelocytic leukemia cell HL-60, with an ED 50 ≤ 25 μg/ml for 2-day treatment. The EtOAc extract induced the characteristic apoptotic symptoms in the HL-60 cells, DNA fragmentation and chromatin condensation, occurring within 6–8 h of treatment at a dose of 200 μg/ml. The activation of caspase-3 and the specific proteolytic cleavage of poly ADP-ribose polymerase were detected during the course of apoptosis induction. These results suggest that the Cs mycelium extract inhibited the cancer cell proliferation by inducing cell apoptosis.
ISSN:0024-3205
1879-0631
DOI:10.1016/j.lfs.2004.05.029