NO donors-relaxation is impaired in aorta from hypertensive rats due to a reduced involvement of K+ channels and sarcoplasmic reticulum Ca2+-ATPase

To examine the vasodilatation induce by the NO donors, [Ru(terpy)(bdq)NO]3+ (TERPY) and sodium nitroprusside (SNP), and to compare their effects in aortic rings from hypertensive 2K-1C and normotensive 2K rats. Vascular reactivity was performed in aortic rings pre-contracted with phenylephrine (Phe...

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Published in:Life sciences (1973) 2011-10, Vol.89 (17-18), p.595-602
Main Authors: Bonaventura, Daniella, de Lima, Renata Galvão, da Silva, Roberto Santana, Bendhack, Lusiane Maria
Format: Article
Language:English
Subjects:
aorta
Ca2-transporting ATPase
cyclic GMP
hypertension
K+ channel
nitric oxide
Nitric oxide donor
nitroprusside
phenylephrine
potassium channels
rats
Renal hypertension
sarcoplasmic reticulum
vasodilation
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Summary:To examine the vasodilatation induce by the NO donors, [Ru(terpy)(bdq)NO]3+ (TERPY) and sodium nitroprusside (SNP), and to compare their effects in aortic rings from hypertensive 2K-1C and normotensive 2K rats. Vascular reactivity was performed in aortic rings pre-contracted with phenylephrine (Phe 100nM). We have analyzed the maximal relaxation (Emax) and potency (pD2) of NO donors. Potency of SNP was greater than TERPY in both arterial groups. The vasodilatation induced by TERPY was greater in 2K than in 2K-1C, and it was inhibited by sGC inhibitor ODQ in 2K and in 2K-1C aortic rings. ODQ did not alter the efficacy to SNP, but it reduced its potency in 2K and 2K-1C. The blockade of K+ channels reduced the potency of TERPY only in aortic rings of 2K. On the other hand, the potency of SNP was reduced in both 2K and 2K-1C. The combination of ODQ and TEA reduced the relaxation induced by TERPY and SNP in 2K and reduced the efficacy to SNP in 2K-1C aortic rings but it had no additional effect on the TERPY relaxation in 2K-1C aortas. The production of cGMP induced by TERPY was greater than that produced by SNP, which was similarly increased in 2K and 2K-1C. Sarcoplasmic reticulum Ca-ATPase inhibition only impaired the relaxation induced by SNP in 2K aortic rings. Taken together, our results provide evidences that in this model of hypertension, impaired K+ channels activation by TERPY and SERCA activation by SNP may contribute to decreased vasodilatation.
ISSN:0024-3205
1879-0631
DOI:10.1016/j.lfs.2011.07.022