The antiproliferative activity of 3-deoxyanthocyanins extracted from red sorghum (Sorghum bicolor) bran through P53-dependent and Bcl-2 gene expression in breast cancer cell line

• Published in: Life sciences (1973) 2013-03, Vol.92 (6-7), p.379-382
• Main Authors: Suganyadevi, P., Saravanakumar, K.M., Mohandas, S.
• Format: Article
• Language: English
• Subjects: 3-deoxyanthocyanins; anthocyanins; apoptosis; bran; Breast cancer cell line; breast neoplasms; cytotoxicity; gene expression; gene expression regulation; genes; humans; messenger RNA; Red sorghum bran; reverse transcriptase polymerase chain reaction; Sorghum bicolor
• ISSN: 0024-3205; 1879-0631
• DOI: 10.1016/j.lfs.2013.01.006

The aim of this study was to investigate the anti proliferative activity of 3-deoxyanthocyanin extracted from red sorghum bran on human breast cancer cell line MCF 7. The confirmatory tests were carried out in vitro through the expression studies of p53 and bcl 2 genes in MCF 7 cells. The 3-deoxyanthocyanins were isolated from red sorghum bran and cytotoxic studies were performed in MCF 7 cell line by MTT assay. The mRNA expression levels of p53 and bcl 2 genes were performed using semi-quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) analysis in MCF 7 cells. On cytotoxic studies, the present data indicates sorghum anthocyanins, which showed 84.09% of inhibition in the proliferation of MCF 7 cells, and the CTC50 value was 300μg/ml. The sorghum 3-deoxyanthocyanins induced apoptosis in MCF 7 was mediated by stimulation of the p53 gene and down regulation of the bcl 2 gene. The significance of our work was the anthocyanin isolated from red sorghum bran inhibits the proliferation of human breast cancer cell line.