Effects of MgSO 4 and magnesium transporters on 6-hydroxydopamine-induced SH-SY5Y cells

• Published in: Life sciences (1973) 2017-03, Vol.172, p.48-54
• Main Authors: Lin, Ling, Ke, Zili, Lv, Meiqi, Lin, Renxi, Wu, Bin, Zheng, Zhihong
• Format: Article
• Language: English
• Subjects: Cell Line, Tumor; Humans; Ion Transport; Magnesium Sulfate - metabolism; Magnesium Sulfate - pharmacology; Oxidopamine - adverse effects
• ISSN: 0024-3205; 1879-0631
• DOI: 10.1016/j.lfs.2016.12.010

The magnesium ion (Mg ) fulfils several important functions for living organisms. We investigated whether there is a protective effect of MgSO on 6-OHDA-induced neurotoxicity in SH-SY5Y cells, and gained insight into the effects of cellular mRNA and protein expression of the magnesium transporters SLC41A1, NIPA1, MagT1 and CNNM2 on 6-OHDA-induced neurotoxicity. The effect of MgSO on cell viability in 6-OHDA-treated SH-SY5Y cells was measured using a CCK-8 kit. The mRNA and protein expression of SLC41A1, NIPA1, MagT1, and CNNM2 were detected using reverse transcription-qPCR and Western blot. The results showed that SH-SY5Y cells treated with 25-50μM 6-OHDA for 24h significantly decreased cell viability, while if pre-incubated with 0.125-1mM MgSO for 1h before adding 6-OHDA it partially prevented the cell damage. There was a significant decrease in cellular mRNA and protein expression of SLC41A1, NIPA1, MagT1 and CNNM2 in 6-OHDA treated SH-SY5Y cells, and MgSO can reverse its decline. Our results suggest that MgSO may protect SH-SY5Y cells against 6-OHDA-induced cell injury and that gene expression of SLC41A1, NIPA1, MagT1, and CNNM2 might be involved in dopaminergic neurons.