Comparison of the developmental competence and quality of bovine embryos obtained by in vitro fertilization with sex-sorted and unsorted semen from seven bulls

In artificial insemination, semen quality is considered to be closely related to the resultant pregnancy rate and the ratio of embryo development into fetus. The aim of this study was to evaluate the quality of sex-sorted versus unsorted semen used for in vitro fertilization (IVF). Semen was collect...

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Bibliographic Details
Published in:Livestock science 2015-11, Vol.181, p.263-270
Main Authors: Liu, Xia, Hu, Tingxi, Sun, Weijun, Hao, Haisheng, Liu, Yan, Zhao, Xueming, Zhu, Huabin, Du, Weihua
Format: Article
Language:English
Subjects:
Apoptosis
Embryonic development
Gene expression
ICM/TCN
Sex-sorted semen
Unsorted semen
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Summary:In artificial insemination, semen quality is considered to be closely related to the resultant pregnancy rate and the ratio of embryo development into fetus. The aim of this study was to evaluate the quality of sex-sorted versus unsorted semen used for in vitro fertilization (IVF). Semen was collected from seven bulls (A)–(G); each sample was divided in two, sex-sorted or not. Following IVF, the fertilization rate, embryonic cleavage rate, blastocyst formation rate, total cells number (TCN) of blastocysts, inner cell mass (ICM)/TCN ratio and apoptotic cell ratio (ACR) were determined. The results showed that except for bull C, the unsorted semen yielded a significantly higher IVF rate than sex-sorted semen (p0.05). However, the GDF9 gene expression in sexed embryos from bull D was significantly higher than in the others (p
ISSN:1871-1413
1878-0490
DOI:10.1016/j.livsci.2015.09.009