Effects of dietary supplementation of linseed oil (Omega-3) on quality parameters of Nili Ravi bull spermatozoa

• Published in: Livestock science 2019-06, Vol.224, p.57-59
• Main Authors: Ahmad, Shafiq, Ali, Shujait, Abbas, Asghar, Abbas, Rao Zahid, Zubair, Muhammad, Raza, Muhammad Asif, Bano, Naheed, Shafi, Muhammd Usman, Badar, Syed Nadeem, Hussain, Kashif, Rizwan, Muhammad, Ahmad, Tanveer
• Format: Article
• Language: English
• Subjects: Linseed oil; Nili Ravi; Omega-3; Semen; Viability
• ISSN: 1871-1413; 1878-0490
• DOI: 10.1016/j.livsci.2019.04.007

•In spite of the advances in cryopreservation of semen, many injuries occur at molecular and cellular level during this process and affect the sperm quality, functions and fertilization potential.•Linseed oil is rich source of Omega-3 fatty acids which can improve semen quality parameters by improving sperm number, their motilty and viability in farm animals.•Dietary supplementation with linseed oil (Omega-3 fatty) showed a positive effect on semen quality parameters of Nilli-Ravi buffalo bulls in terms of integrity of plasma membrane, acrosome and also an increase in the percentage of live spermatozoa was observed. Current experiment was designed to evaluate the effects of dietary supplementation of linseed oil (Omega-3) on the post thaw quality parameters of Nili Ravi buffalo bull's semen. For this purpose, twelve adult Nili Ravi buffalo bulls were randomly divided into three equal groups i.e. A, B and C. Groups A and B were fed with linseed oil at125 ml and 250 ml/day, respectively for 12 weeks. Group-C served as control group. Semen samples (two ejaculates/bull) were collected with the help of artificial vagina at different weeks of experiment. Semen samples were thawed and evaluated for different parameters such as percent motility of spermatozoa, percent live spermatozoa, plasma membrane and acrosome integrity. Statistical analysis of data revealed that supplementation of ration with linseed oil at125 ml and 250 ml for 12 weeks to the bulls of groups A and B showed significantly higher (P  0.05) was found between groups A and B. Post thaw plasma membrane integrity and acrosome integrity were also not significantly different among linseed oil supplemented groups. It was concluded from study that supplementation of ration with linseed oil has positive impact on quality parameters of spermatozoa.