Evaluation of a serum-free culture medium for the enhanced vitrification cryosurvival of bovine in vitro-derived embryos

• Published in: Livestock science 2022-06, Vol.260, p.104922, Article 104922
• Main Authors: de Camargo, Janine, Rodrigues, Rafaela, Valente, Roniele Santana, Muller, Diego Borba, Vireque, Alessandra Aparecida, Belaz, Katia Roberta Anacleto, Bohrer, Rodrigo Camponogara, Basso, Andrea Cristina, Eberlin, Marcos Nogueira, Fontes, Patricia Kubo, Nogueira, Marcelo Fábio Gouveia, Sudano, Mateus José
• Format: Article
• Language: English
• Subjects: Bovine embryo; Cryosurvival; Lipids; Vitrification
• ISSN: 1871-1413; 1878-0490
• DOI: 10.1016/j.livsci.2022.104922

•The designed medium to enhance embryo cryosurvival can increase the use of IVP embryos for cattle reproductive management in large-scale commercial programs.•The embryos produced in vitro using the designed medium to enhance cryosurvival presented the closest lipid profiles compared with the in vivo counterparts.•The MB medium-induced cryosurvival can be explained by embryonic lipid characteristics and increased cell number. This is a feasibility study aimed to determine the effectiveness of a culture media designed to enhance embryonic cryopreservation based on laboratory-accessed quality phenotypes of in vitro produced bovine embryos. Recovered bovine oocytes were matured and fertilized in vitro and the presumptive zygotes that were obtained were randomly distributed into three culture media: (i) Control (C): modified synthetic oviduct fluid (mSOF) culture medium supplemented with 5 mg/mL BSA and 2.5% fetal calf serum; (ii) Medium A (MA): serum-free altered mSOF medium A; and iii) Medium B (MB): serum-free altered mSOF medium B. Embryos cultured in MA and MB presented a better viability after cryopreservation (P  0.05, MB only) when compared to C. The MB-derived blastocysts had the highest (P