High performance liquid chromatographic separation of interesterified palm oil with tributyrin

Short-chain triacylglycerol, tributyrin (glyceryl- sn-1,2,3-tri-butyric acids), was interesterified with palm oil to produce mixtures of structured triacylglycerol (SL-TAG) species as a low-calorie lipid. Lipozyme RM IM from Rhizomucor miehei, known as a 1,3-regioselective immobilized lipase, was us...

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Bibliographic Details
Published in:Food science & technology 2008-01, Vol.41 (8), p.1446-1451
Main Authors: Lee, Jeung H., Jones, Kerby C., Foglia, Thomas A., Lee, Cho-Rong, Akoh, Casimir C., Lee, Ki-Teak
Format: Article
Language:English
Subjects:
APCI mass spectrometry
Biological and medical sciences
esterification
Evaporated light-scattering detection
Fat industries
Food industries
Fundamental and applied biological sciences. Psychology
General aspects
High performance liquid chromatography
Interesterification
low calorie foods
Methods of analysis, processing and quality control, regulation, standards
Palm oil
palm oils
separation
structured lipids
Tributyrin
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Summary:Short-chain triacylglycerol, tributyrin (glyceryl- sn-1,2,3-tri-butyric acids), was interesterified with palm oil to produce mixtures of structured triacylglycerol (SL-TAG) species as a low-calorie lipid. Lipozyme RM IM from Rhizomucor miehei, known as a 1,3-regioselective immobilized lipase, was used as a catalyst. During 24 h reaction, the reaction mixture was analyzed with Hypersil ®BDSCPS high performance liquid chromatography (HPLC) column to determine neutral lipids composition. Also, the compositional changes of TAG, as well as monoacylglycerol (MAG)/diacylglycerol (DAG) as by-products, were determined. After 24 h, 5.8 g/100 g DAG was found in the reaction mixture, while 89.5 g/100 g TAG was observed. The reaction mixture was also analyzed with Nova-Pak ® C18 and Chrompack Si60 columns for separation of SL-TAG species. It was found that the normal-phase (NP)-HPLC with Chrompack Si60 column readily separated SL-TAG species composed of short- and long-chain acyl residues. Further, mass spectrometer was employed for identifying the separated SL-TAG molecules. SL-TAG containing two butyryl and one long-chain acyl residue (e.g., PBB and OBB) were most abundant in the interesterified product.
ISSN:0023-6438
1096-1127
DOI:10.1016/j.lwt.2007.08.021