Reactivity of acrylamide with coffee melanoidins in model systems

• Published in: Food science & technology 2012-03, Vol.45 (2), p.198-203
• Main Authors: Pastoriza, Silvia, Rufián-Henares, José Ángel, Morales, Francisco J.
• Format: Article
• Language: English
• Subjects: Acrylamide; acrylamides; amino acids; Biological and medical sciences; cellulose; Coffee; Coffee, tea and other stimulative beverage industries; dose response; Food industries; Food toxicology; Fundamental and applied biological sciences. Psychology; Maillard reaction; Melanoidins; roasting; sand; temperature
• ISSN: 0023-6438; 1096-1127
• DOI: 10.1016/j.lwt.2011.08.004

Coffee and its substitutes have been described as complex matrices for acrylamide (ACR) analysis due to both analytical interferences and ACR instability in the matrix. Melanoidins are multifunctional and biochemically active polymers which are formed in large extent during coffee roasting. Model systems composed of ACR (elimination studies) or glucose-asparagine (ACR formation/elimination studies) with/without melanoidins was heated at 180 °C. Washed sea sand and cellulose microcrystalline were used as matrix. Coffee melanoidins had a direct influence on the fate of ACR under heating, while the effect was not observed at room temperature. In addition, ACR decrease was also related to the reaction time and the initial amount of melanoidins in the media, where clearly a dose-response was observed. In contrast, pH (from 3.5 to 7.0) had no significant effect on ACR reactivity towards melanoidins. It is hypothesized that nucleophilic amino groups of amino acids from the proteinaceous backbone of melanoidins react via the Michael addition reaction with ACR, although the exact mechanism is unknown. Then, melanoidins could modulate the reaction pathways of ACR formation and elimination during coffee roasting and serve as acrylamide-mitigation substance. ► We investigated the effect of coffee melanoidins over acrylamide in model systems. ► Melanoidins influenced the formation/elimination of acrylamide (ACR) under heating. ► Such influence followed a dose-response model. ► We hypothesized that the proteinaceous backbone of melanoidins reacts with ACR. ► This reaction involves nucleophilic aminoacids and ACR via Michael addition reaction.