Development of enzymic time-temperature integrators with rapid detection for evaluation of continuous HTST pasteurization processes

• Published in: Food science & technology 2012-06, Vol.47 (1), p.110-116
• Main Authors: Aguiar, Helena de Fazio, Yamashita, André Shigueo, Gut, Jorge Andrey Wilhelms
• Format: Article
• Language: English
• Subjects: Biological and medical sciences; Enzyme inactivation; Food engineering; Food industries; Fundamental and applied biological sciences. Psychology; General aspects; Kinetic model; Methods of analysis, processing and quality control, regulation, standards; Pasteurization; Time temperature integrator
• ISSN: 0023-6438; 1096-1127
• DOI: 10.1016/j.lwt.2011.12.027

The assessment of the thermal process impact in terms of food safety and quality is of great importance for process evaluation and design. This can be accomplished from the analysis of the residence time and temperature distributions coupled with the kinetics of thermal change, or from the use of a proper time-temperature integrator (TTI) as indicator of safety and quality. The objective of this work was to develop and test enzymic TTIs with rapid detection for the evaluation of continuous HTST pasteurization processes (70–85 °C, 10–60 s) of low-viscosity liquid foods, such as milk and juices. Enzymes peroxidase, lactoperoxidase and alkaline phosphatase in phosphate buffer were tested and activity was determined with commercial reflectometric strips. Discontinuous thermal treatments at various time-temperature combinations were performed in order to adjust a first order kinetic model of a two-component system. The measured time-temperature history was considered instead of assuming isothermal conditions. Experiments with slow heating and cooling were used to validate the adjusted model. Only the alkaline phosphatase TTI showed potential to be used for the evaluation of pasteurization processes. The choice was based on the obtained z-values of the thermostable and thermolabile fractions, on the cost and on the validation tests. ► Enzymic based TTIs with rapid detection proposed for evaluation of HTST processes. ► Two-component 1st order kinetic model adjusted with full time-temperature histories. ► Best TTI obtained was alkaline phosphatase in phosphate buffer.