Unpurified Gelidium-extracted carbohydrate-rich fractions improve probiotic protection during storage

• Published in: Food science & technology 2018-10, Vol.96, p.694-703
• Main Authors: Alehosseini, Ali, Gomez del Pulgar, Eva-María, Gómez-Mascaraque, Laura G., Martínez-Sanz, Marta, Fabra, Maria José, Sanz, Yolanda, Sarabi-Jamab, Mahboobe, Ghorani, Behrouz, Lopez-Rubio, Amparo
• Format: Article
• Language: English
• Subjects: Agarose; Encapsulation; Internal gelation; Probiotics; Whey protein concentrate
• ISSN: 0023-6438; 1096-1127
• DOI: 10.1016/j.lwt.2018.06.043

The objective of this work was to investigate whether the use of unpurified agar-based fractions extracted from the seaweed Gelidium as microencapsulation matrices has an impact on probiotic protection during storage. Therefore, unpurified and pure agar and agarose-based microcapsules were produced through emulsification/internal gelation for the protection of Bifidobacterium pseudocatenulatum CECT 7765. Initially, agarose-based formulations with other biopolymers were evaluated, given the excellent oxygen barrier properties of this polysaccharide. The optimal combination in terms of probiotic protection was selected for further experiments and this agarose-based formulation was compared with microcapsules produced using both pure and unpurified agar-based fractions. The presence of other compounds (mainly proteins and polyphenols) in the unpurified agar fractions significantly improved the viability of these sensitive probiotic bacteria both at ambient and refrigerated storage conditions. Furthermore, the presence of impurities allowed the increase of solids content in the formulation giving raise to stronger gel particles, which could contribute to limited oxygen diffusion, thus, partly explaining the improved protection. Therefore, this work demonstrates the potential of more cost-effective less purified carbohydrate-based fractions for probiotic protection. •Agarose and agar-based matrices were used for encapsulation of B. pseudocatenolanum.•Unpurified agar containing proteins and polyphenols was also evaluated.•The capsules were obtained through an emulsification/internal gelation process.•Probiotic viability in the different systems was evaluated during storage.•Unpurified agar fractions significantly improved probiotic protection.