Evaluating of quality of rice bran protein concentrate prepared by a combination of isoelectronic precipitation and electrolyzed water treatment

A combination of isoelectronic precipitation and electrolyzed water treatment (IP-EWT) process was use for simultaneous recovery of protein and phosphorus compounds from heat-stabilized defatted rice bran (HSDFRB). The highest protein content (65.1 w/w%) recovery ratio (over 50%) of protein concentr...

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Bibliographic Details
Published in:Food science & technology 2019-01, Vol.99, p.262-267
Main Authors: Watanabe, Masanori, Yamada, Chikako, Maeda, Isamu, Techapun, Charin, Kuntiya, Ampin, Leksawasdi, Noppol, Seesuriyachan, Phisit, Chaiyaso, Thanongsak, Takenaka, Shinji, Shiono, Tadahiko, Nakamura, Kozo, Endo, Shujiro
Format: Article
Language:English
Subjects:
Allergenic proteins
Defatted rice bran
Electrolyzed water
Isoelectric precipitation
Rice protein
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Summary:A combination of isoelectronic precipitation and electrolyzed water treatment (IP-EWT) process was use for simultaneous recovery of protein and phosphorus compounds from heat-stabilized defatted rice bran (HSDFRB). The highest protein content (65.1 w/w%) recovery ratio (over 50%) of protein concentrate were attained by two-stage protein extraction with IP-EWT. Analysis of amino-acid composition demonstrated excellent amino acid value (76.6%) in the protein fraction than those of commercial cereals such as rice (61%), wheat (39%), and corn (31%). In addition, according to SDS-PAGE and immunoblotting analysis, neither rice allergenic protein nor heavy metals were detected in either HSDFRB or protein fractions extracted by IP-EWT. These results suggest that as an eco-friendly process, this combined (IP-EWT) process is suitable for practical recovery of highly concentrated and safe protein from HSDFRB without using enzymes or chemicals such as organic solvents, buffering agents, and surfactants. •Rice protein concentrate was recovered and purified by using IP-EWT process.•Excellent amino acid value (76.6%) was indicated in the protein fraction.•Allergenic protein were not detected in HSDFRB and protein fractions.•IP-EWT process is suitable for recovery of highly concentrated and safe rice protein.
ISSN:0023-6438
1096-1127
DOI:10.1016/j.lwt.2018.09.059