Effect of hydrogen peroxide on the dehydrogenase and quinone-reductase activity of irradiated Lactobacillus plantarum cells

The resistance of lactobacilli to oxidative stress is of great importance for their applicability as probiotics. This study aimed to evaluate the response of Lactobacillus plantarum strain ATCC® 14917™, grown in either de Man, Rogosa, and Sharpe agar (MRS medium) or tryptic soy broth (TSB medium), t...

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Bibliographic Details
Published in:Food science & technology 2020-12, Vol.134, p.110236, Article 110236
Main Authors: Kalneniece, Kristine, Bankovskis, Vasilijs, Selga, Turs, Kusins, Toms, Balode, Inga, Mazkalnina, Liva, Shvirksts, Karlis, Grube, Mara, Kizane, Gunta, Grinbergs, Andrejs, Muter, Olga
Format: Article
Language:English
Subjects:
Cell morphology
De man
Gamma irradiation
Oxidative stress
Quinone reductase
Rogosa and sharpe
Tryptone soya broth
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Summary:The resistance of lactobacilli to oxidative stress is of great importance for their applicability as probiotics. This study aimed to evaluate the response of Lactobacillus plantarum strain ATCC® 14917™, grown in either de Man, Rogosa, and Sharpe agar (MRS medium) or tryptic soy broth (TSB medium), to 1–2 mM H2O2 after the exposure to different doses of ionising radiation. Two bacterial extracellular enzyme groups, dehydrogenases (DHAs) and quinone reductases (QRs), served as the criteria of viability and antioxidant activity, respectively. The irradiated L. plantarum culture grown in TSB showed increased QR activity at irradiation doses of 2–50 Gy, with the maximum activity at 10 Gy, compared to non-irradiated cells. The QR activity of the MRS-grown culture was increased significantly (p 
ISSN:0023-6438
1096-1127
DOI:10.1016/j.lwt.2020.110236