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Antibacterial activities and mechanisms of vine tea extract and 2R, 3R-Dihydromyricetin on Escherichia coli

In this study, the antibacterial mechanisms of vine tea aqueous extract (VTE) and 2R, 3R-Dihydromyricetin (DMY) against Escherichia coli (E. coli) were investigated. The results showed they had good antibacterial activities on E. coli, and affected cell membrane and nucleoid. The minimum bactericida...

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Bibliographic Details
Published in:Food science & technology 2021-07, Vol.146, p.111393, Article 111393
Main Authors: Cui, Shu-Mei, Li, Ting, Liang, Hai-yun, He, Ke-Ke, Zheng, Yu-Mei, Tang, Meng, Ke, Chong-rong, Song, Li-Ya
Format: Article
Language:English
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Summary:In this study, the antibacterial mechanisms of vine tea aqueous extract (VTE) and 2R, 3R-Dihydromyricetin (DMY) against Escherichia coli (E. coli) were investigated. The results showed they had good antibacterial activities on E. coli, and affected cell membrane and nucleoid. The minimum bactericidal concentrations of VTE and DMY were 3.2 and 1.25 mg/mL, respectively. The constituent leakage tests showed VTE and DMY caused significant increases of extracellular alkaline phosphatase (24.4 ± 0.4% and 5.3 ± 0.1%) and β-Galactosidase (27.6 ± 1.0% and 13.6 ± 0.5%), indicating the membrane integrity might be damaged. The lipids studies showed the ratio of unsaturated fatty acids to saturated fatty acids in treated groups were significant decrease (P 
ISSN:0023-6438
1096-1127
DOI:10.1016/j.lwt.2021.111393