Phenotypic and probiotic characterization of isolated LAB from Himalayan cheese (Kradi/Kalari) and effect of simulated gastrointestinal digestion on its bioactivity

In the present study, LAB (Lactic acid bacteria) were isolated and identified from Kradi cheese. Results revealed presence of five dominant LAB viz. Lb. curvatus, Lb. sake, Lb. planatrum, Pediococcus pentosaceous and Enterococcus faecium. Among these probiotic strains, Lb. planatrum showed highest h...

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Bibliographic Details
Published in:Food science & technology 2021-09, Vol.149, p.111669, Article 111669
Main Authors: Mushtaq, Mehvesh, Gani, Adil, Noor, Nairah, Masoodi, F.A.
Format: Article
Language:English
Subjects:
Bioactive peptides
Functional foods
Kradi cheese
Probiotics
Simulated digestion
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Summary:In the present study, LAB (Lactic acid bacteria) were isolated and identified from Kradi cheese. Results revealed presence of five dominant LAB viz. Lb. curvatus, Lb. sake, Lb. planatrum, Pediococcus pentosaceous and Enterococcus faecium. Among these probiotic strains, Lb. planatrum showed highest hydrophobicity and antimicrobial activity against different pathogenic strains. The cheese sample was further subjected to simulated gastrointestinal digestion (SGID) to obtain water-soluble peptide extract (WSPE). Bioactivity assays of WSPE, including, ABTS radical inhibition, reducing power, lipid peroxidation inhibition, α-glucosidase inhibition and α-amylase inhibition increased significantly from 12.03 to 58.89%, 14.85–55.72%, 14.67–52.43%, 15.55–57.65%, 17.88 %-59.33%, respectively from 0 to 420 min of digestion. Anti-cancer and anti-hypertensive activity of WSPE increased significantly from 13.44 to 69.39% and 19.75–71.23% upon digestion in simulated gastrointestinal juice (SGIJ). The overall results suggest that bioactivity of sample enhanced upon digestion validating Kradi cheese as a potential source of bioactive peptides and probiotics and therefore, may be categorized as a functional food. •L. curvatus, L. sake, L. planatrum, P. pentosaceous and E. faecium were isolated from Kradi cheese.•Lb. planatrum showed highest hydrophobicity and antimicrobial activity.•Release of bioactive peptides increased upon simulated digestion.•Bioactivity of cheese increased upon simulated digestion.•Kradi is cheese with functional properties.
ISSN:0023-6438
1096-1127
DOI:10.1016/j.lwt.2021.111669