Characterization and film-forming properties of acid soluble collagens from different by-products of loach (Misgurnus anguillicaudatus)

In this study, acid soluble collagens (ASCs) from different by-products of loach including skin, head, fins and bone were isolated, characterized and compared. As a result, the highest yield (on wet weight basis) was obtained for ASC from skin (ASC-S, 9.73%) compared with those from fins (ASC-F, 2.0...

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Bibliographic Details
Published in:Food science & technology 2021-09, Vol.149, p.111844, Article 111844
Main Authors: Sun, Peng-peng, Ren, Yuan-yuan, Wang, Si-yao, Zhu, Hong, Zhou, Jue-jun
Format: Article
Language:English
Subjects:
Acid soluble collagens
By-products
Characterization
Film-forming properties
Loach (Misgurnus anguillicaudatus)
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Summary:In this study, acid soluble collagens (ASCs) from different by-products of loach including skin, head, fins and bone were isolated, characterized and compared. As a result, the highest yield (on wet weight basis) was obtained for ASC from skin (ASC-S, 9.73%) compared with those from fins (ASC-F, 2.01%), head (ASC-H, 1.12%) and bone (ASC-B, 0.84%). By SDS-PAGE, these collagens were classified as type I collagens with slight difference of molecular weight. Glycine was the major amino acid in all ASCs with relatively high contents of alanine, hydroxyproline and proline. Fourier transform infrared spectroscopy indicated that triple helical structures of all ASCs were well preserved and scanning electron microscope exhibited fibrillary structure of ASC-B while sheet structure in others. Thermal stability of ASCs was determined by differential scanning calorimetry, rheometer and thermogravimetric (TG), respectively. It was concluded that all ASCs had different maximum transition (Tmax), denaturation (Td) and onset decomposition (To) temperatures. Besides, film-forming properties of ASCs from loach were also evaluated with ASC-F film showing strong tensile strength, best extensibility and barrier property compared with others, which suggested that ASCs from different by-products of loach had tremendous potential for applications in food packaging and coating in future. •Acid soluble collagens (ASCs) were isolated from different by-products of loach.•Less intra- and inter-molecular crosslink existed in ASC-F.•Denaturation and thermal decomposition temperatures of ASCs were not identical.•ASC-F could form film with strong mechanical and barrier property.
ISSN:0023-6438
1096-1127
DOI:10.1016/j.lwt.2021.111844