Application of 1H-NMR- and LC-MS based Metabolomic analysis for the evaluation of celery preservation methods

In this study, 1H NMR spectroscopy coupled with multivariate analyses was used to evaluate the effectiveness of celery preservation techniques. The extraction parameters were optimised to achieve high recoveries of the majority (9 out of 14) of the quantified metabolites, and to reduce fluctuations...

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Bibliographic Details
Published in:Food science & technology 2022-11, Vol.169, p.113938, Article 113938
Main Authors: Lau, Hazel, Heng Tan, Lai, Ying Ee, Liang, Dayal, Hiranya, Ying Lim, Si, Liu, Feng, Yau Li, Sam Fong
Format: Article
Language:English
Subjects:
Nanocellulose
Senescence
Storage
Vegetable
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Summary:In this study, 1H NMR spectroscopy coupled with multivariate analyses was used to evaluate the effectiveness of celery preservation techniques. The extraction parameters were optimised to achieve high recoveries of the majority (9 out of 14) of the quantified metabolites, and to reduce fluctuations in concentrations (within relative standard deviation of 3.5%) and average chemical shifts (0.011 ppm). Subsequently, the optimised method was applied to evaluate metabolite changes caused by different preservation treatments over a 14-day storage period. Nanocellulose treatment (5 g/L) was ascertained to be the most effective from untargeted and targeted principal component analysis (PCA) results, correlating with visual findings, and the significantly reduced metabolite variations (p 
ISSN:0023-6438
DOI:10.1016/j.lwt.2022.113938