Development of a three-dimensional graphene-based photoelectrochemical biosensor and its use for monitoring lipase activity

• Published in: Food science & technology 2022-12, Vol.170, p.114076, Article 114076
• Main Authors: Wang, Liqi, Zhang, Hairong, Li, Ziyue, Qu, Jiayao, Xing, Kaiwen, Wang, Minghao, Han, Cuiping, Qiu, Zeguo, Yu, Dianyu
• Format: Article
• Language: English
• Subjects: Detection; Lipase activity; Photoelectrochemical biosensor; Three-dimensional graphene oxide
• ISSN: 0023-6438; 1096-1127
• DOI: 10.1016/j.lwt.2022.114076

In recent years, photoelectrochemical (PEC) biosensors have shown great application prospects in biological analysis and food detection. In this work, we developed and tested a new PEC biosensor. Three-dimensional graphene oxide (3DGO) was generated and deposited on an indium tin oxides (ITO) electrode. The photoelectrically active poly[Nile blue] (PNb) was formed on this electrode by electropolymerization, and glycerol dehydrogenase (GDH) was immobilised to generate the GDH-PNb-3DGO-ITO biosensor. This newly developed PEC biosensor exhibited excellent photoelectric response to lipase activity under light conditions, with a detection range of 0.1–6 U/mL and a lower detection limit of 0.069 U/mL. When the GDH-PNb-3DGO-ITO sensor was used for PEC detection of lipase activity by the standard addition recovery method, the recovery rate of the standard addition was 98.68–105.41%, and the relative standard deviation (RSD) was 2.1–3.5%, with good storage stability and repeatability. Therefore, this work provides new ideas for the design and preparation of PEC biosensors and introduces a new detection method for monitoring lipase activity. [Display omitted] •Development of a PEC biosensor to detect lipase activity.•The fabricated biosensor has excellent optoelectronic properties.•The sensor has a low detection limit (0.069 U/mL), providing a platform for lipase activity detection.