Efficacy of triple-wash using a peroxyacetic acid and hydrogen peroxide solution at reducing populations and cross-contamination of Salmonella Typhimurium and the surrogate Enterococcus faecium on tomatoes

The efficacy of a triple-wash system to reduce and mitigate cross-contamination of Salmonella Typhimurium and Enterococcus faecium was tested on tomatoes. Tomatoes were dip-inoculated with S. Typhimurium or E. faecium followed by triple-washing for 45 s at each wash step: water + antimicrobial + wat...

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Bibliographic Details
Published in:Food science & technology 2023-02, Vol.175, p.114499, Article 114499
Main Authors: Stearns, Rebecca, Coe, Corey, Holásková, Ida, Matak, Kristen, Freshour, Annette, Jaczynski, Jacek, Xue, Jingyi, Luo, Yangchao, Jones, Lisa, Wang, Xinhao, Shen, Cangliang
Format: Article
Language:English
Subjects:
E. faecium
Peroxyacetic acid
Salmonella
Tomatoes
Triple-wash
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Summary:The efficacy of a triple-wash system to reduce and mitigate cross-contamination of Salmonella Typhimurium and Enterococcus faecium was tested on tomatoes. Tomatoes were dip-inoculated with S. Typhimurium or E. faecium followed by triple-washing for 45 s at each wash step: water + antimicrobial + water (WAW) or water + water + antimicrobial (WWA). A mixture [23 ml/dL hydrogen peroxide + 5.3 ml/dL peroxyacetic acid (SaniDate-5.0, SD)] was tested at 0, 0.0064, 0.1, and 0.25 ml/dL. Microbial population was estimated using a modified most-probable-number (MPN) method. Reductions of S. Typhimurium were similar (P > 0.05) to E. faecium (1.83–3.53 vs 1.72–3.65 log10MPN/g) with the greatest reductions at 0.25 ml/dL of SD. No differences (P > 0.05) were seen in reductions of S. Typhimurium or E. faecium regardless of wash strategy. Application of 0.25 ml/dL of SD in WAW or WWA wash strategy resulted in the lowest (P  0.05) in of survival between S. Typhimurium and E. faecium after treatment with the triple-wash strategies. Results suggest that E. faecium could be an acceptable surrogate for S. Typhimurium when validating antimicrobial washing systems on tomatoes. •There were no differences between WAW and WWA for reducing bacteria on tomatoes.•The triple-wash method with 0.25% SD minimizes bacterial cross-contamination on tomatoes.•E. faecium is an acceptable surrogate for Salmonella Typhimurium on tomatoes.
ISSN:0023-6438
1096-1127
DOI:10.1016/j.lwt.2023.114499