A lateral flow immunochromatographic strip based on RPA and quantum dot nanobeads for rapid identification of pathogenic Yersinia enterocolitica

• Published in: Food science & technology 2023-09, Vol.187, p.115271, Article 115271
• Main Authors: Wang, Han, Zheng, Yu, Ren, Hong-Lin, Xiao, Yi-Ran, Wang, Cong, Chang, Jiang, Guo, Yu-Xi, Hu, Pan, Li, Yan-Song, Liu, Zeng-Shan, Lu, Shi-Ying
• Format: Article
• Language: English
• Subjects: Fluorescence immunochromatographic strips; Pathogenic Yersinia enterocolitica; Quantum dot nanobeads; Recombinase polymerase amplification
• ISSN: 0023-6438; 1096-1127
• DOI: 10.1016/j.lwt.2023.115271

To detect pathogenic Yersinia enterocolitica in meat, a lateral flow immunochromatographic strip targeting on virulence gene ail was developed, which was based on recombinase polymerase amplification (RPA) and quantum dot nanobeads (QDNBs). Under optimal conditions, the minimum detection limit of the strip was 9.6 × 103 CFU/mL, with a standard curve equation of y = 0.144x - 0.5098 (R2 = 0.9863), and the detection times including the RPA reaction at hand temperature (35–36.5 °C), could be controlled in 10 min. When testing spiked sample, the recoveries (%) of Yersinia enterocolitica at 9.6 × 104 CFU/mL were 44.27 ± 2.08 and 45.31 ± 3.12 in beef and pork samples and was 107.55 ± 1.30 in chicken samples. When testing 15 actual samples, only one pork sample was tested positive, consistent with polymerase chain reaction (PCR) results. The strips are body temperature-triggered and rapidly visualized, suitable for rapid field detection of pathogenic Yersinia enterocolitica. [Display omitted] •An immunochromatographic strip was developed to detect Yersinia enterocolitica.•Using antibody conjugated quantum dot nanobeads as fluorescence reporters.•The genome was amplified by recombinase polymerase amplification.•The LOD was 9.6 × 103 CFU/mL with y = 0.144x - 0.5098 (R2 = 0.9863).