Age decreased steady-state concentrations of genistein in plasma, liver, and skeletal muscle in Sprague–Dawley rats

• Published in: Mechanisms of ageing and development 2006-04, Vol.127 (4), p.344-348
• Main Authors: Chen, Chung-Yen, Bakhiet, Raga M.
• Format: Article
• Language: English
• Subjects: Age; Aging; Animals; Anticarcinogenic Agents - pharmacology; Biological and medical sciences; Body Weight; Cardiovascular Diseases - drug therapy; Development. Metamorphosis. Moult. Ageing; Dose-Response Relationship, Drug; Fundamental and applied biological sciences. Psychology; Genistein; Genistein - pharmacology; Glycine max; Lipid Metabolism; Liver; Liver - drug effects; Liver Extracts - metabolism; Male; Models, Statistical; Muscle, Skeletal - drug effects; Muscle, Skeletal - metabolism; Plasma; Rats; Rats, Sprague-Dawley; Skeletal muscle; Soy; Time Factors; Vertebrates: anatomy and physiology, studies on body, several organs or systems
• ISSN: 0047-6374; 1872-6216
• DOI: 10.1016/j.mad.2005.12.003

Soy isoflavones are associated with low incidence of cardiovascular diseases (CVD) and hormone-dependent cancers, but no solid information is available on the relative deposition of isoflavones in the body as a function of age. One-year-old (adult) male Sprague–Dawley rats were fed control diet or one of three high-genistein isoflavone (HGI) diets at a dose of 62, 154, or 308 genistein mg/kg (ppm) diet for 5 weeks; 2-year-old (old) were fed a dose of 154 or 308 ppm. Steady-state genistein concentrations in plasma, liver, and gastrocnemius muscle of the adult rats after 12 h fast revealed a linear dose-dependent manner ( P ≤ 0.0001). However, there was no such relationship in the old rats. Nevertheless, old rats fed the 308 ppm genistein diet had significantly lower steady-state genistein concentrations in plasma and liver than the adult rats did ( P ≤ 0.05); but similar genistein concentration in muscle. The results of this study indicate that steady-state genistein concentrations in tissues of adult rats after 12 h fast exhibited a dose-dependent fashion and were diminished in specific tissues by age.