Isolation and studies on zinc removal using microorganism from contaminated soil

• Main Authors: Jayan, Neethu, Laxmi Deepak Bhatlu, M.
• Format: Conference Proceeding
• Language: English
• Subjects: Bioaccumulation; Bioremediation; Environmental contamination; Heavy metal resistant microorganisms; Heavy metals
• ISSN: 2214-7853; 2214-7853
• DOI: 10.1016/j.matpr.2020.12.071

Heavy metal discharge from various sources creates tremendous dangerous effects among all living creatures. Heavy metals are harmful even at a lower concentration. The technical limitation eliminating heavy metals at low concentration gained the attention of new technology using living microbes. Bioremediation can be used to remove these heavy metals even at lower concentration with the help of living microbes which is not possible using conventional chemical methods. In this work, Zinc resistant microorganism was isolated from the contaminated soil. Employing various staining, morphological and biochemical test the isolated Zinc resistant microorganism was identified as Pseudomonas sp. This isolated microorganism showed resistance up to 1000 ppm of Zinc. Growth optimum conditions obtained for the isolated microorganism were at 35 °C, at pH of 8, when shaken at 200 rpm for a contact time of 30 min. Zinc removal was obtained maximum at 35 °C, pH of 6, at 200 rpm and at a contact time of 60 min. The isolate showed resistance to multiple metals also. FTIR results showed that alkenes, nitro compounds, amino groups are the predominant contributors in Zinc uptake by microorganisms.