Preparation and characterization of Andrographis paniculata extract loaded onto chitosan-based nanoparticles

• Published in: Materials today : proceedings 2024, Vol.110, p.106-109
• Main Authors: Hasham, Rosnani, Yushan Ng, Melissa, Sabtu, Rahimah, Khairul Hafiz Idris, Mohamad, Abdul Rahman, Illani, Yaakob, Harisun, Abdul Hamid, Mariani, Adrus, Nadia, Jamaluddin, Jamarosliza
• Format: Article
• Language: English
• Subjects: Andrographis paniculate; Characterization; Chitosan; Nanoparticles; Preparation
• ISSN: 2214-7853; 2214-7853
• DOI: 10.1016/j.matpr.2023.10.006

Andrographis paniculata (AP) is a multipurpose medicinal herb native to Malaysia with prominent anticancer properties. The main obstacles to its clinical value are ineffective drug loading and release. Thus, this study aimed to screen and characterize the important variables of AP extract loaded onto chitosan nanoparticles to synthesize nanoparticles of a specified size. Nine formulations were prepared and tested for their particle size, polydispersity index, and encapsulation efficiency by altering the mass ratio of chitosan to tripolyphosphate (2:1, 4:1, and 6:1) and reaction duration (30 min, 60 min, and 90 min). The most promising formulation was then examined for zeta potential, shape, and stability. The optimum formulation had a size of 79.68 ± 0.30 nm, a PDI of 0.200 ± 0.013, and an encapsulation efficiency of 81.71 ± 1.486 %. The selected formulation's zeta potential was measured at −4.60 ± 0.711 mV, and the micrographs revealed spherical and smooth-surfaced nanoparticles in dispersion. These findings indicate that the nanoparticles prepared were able to acquire relatively tiny sizes below 600 nm under specified conditions.