Approaches to characterize extended spectrum beta-lactamase/beta-lactamase producing Escherichia coli in healthy organized vis-a-vis backyard farmed pigs in India

The study was undertaken to investigate the occurrence and to characterize the ESBL/beta-lactamase producing-Escherichia coli in healthy pigs of organized and backyard farms in West Bengal, India. Total 200 rectal swabs were collected randomly from healthy pigs maintained in four organized farms and...

Full description

Saved in:
Bibliographic Details
Published in:Infection, genetics and evolution genetics and evolution, 2015-12, Vol.36, p.224-230
Main Authors: Samanta, Indranil, Joardar, Siddhartha N., Mahanti, Achintya, Bandyopadhyay, Samiran, Sar, Tapas K., Dutta, Tapan K.
Format: Article
Language:English
Subjects:
Agriculture
Animals
beta-Lactam Resistance - genetics
beta-Lactamases - genetics
Carrier State - epidemiology
Carrier State - microbiology
Carrier State - veterinary
E. coli
ESBL
Escherichia coli - enzymology
Escherichia coli - genetics
Escherichia coli Infections - epidemiology
Escherichia coli Infections - microbiology
Escherichia coli Infections - veterinary
Healthy
India
India - epidemiology
Pig
Rectum - microbiology
Swine
West Bengal
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The study was undertaken to investigate the occurrence and to characterize the ESBL/beta-lactamase producing-Escherichia coli in healthy pigs of organized and backyard farms in West Bengal, India. Total 200 rectal swabs were collected randomly from healthy pigs maintained in four organized farms and 10 backyard farms (n=100 each) and 76 isolates were identified as E. coli from organized (48/100, 48%) and backyard pigs (28/100, 28%). Twelve E. coli isolates (6%) in the present study were detected to possess any of the ESBL/beta-lactamase genes studied. ESBL/beta-lactamase producers were isolated with significantly more frequency from backyard pigs than the organized farm pigs (p=0.026). Six of ESBL/beta-lactamase producing isolates were phenotypically confirmed as CTX-M producers and ten of them were confirmed as TEM/SHV producers. PCR and sequencing of the amplified product from representative isolates revealed the presence of blaCTX-M-9, blaSHV-12 and blaTEM-1. No unique combination of the studied beta lactamase genes for organized and backyard farm pig isolates was noted. The ESBL isolates belonged to O13, O55, O133, O153, O157, O158, O166, rough and OUT serogroups. The association of heat labile toxin (elt) (p
ISSN:1567-1348
1567-7257
DOI:10.1016/j.meegid.2015.09.021