Effect of Paraoxonase1 (PON1) gene polymorphisms on PON1 activity, HDL, LDL and MDA levels in women with polycystic ovary syndrome (PCOS): A case-control study

• Published in: Meta Gene 2019-06, Vol.20, p.100552, Article 100552
• Main Authors: Jeelani, Humira, Ganie, Mohd Ashraf, Amin, Shajrul, Fatima, Qudsia, Kawa, Iram Ashaq, Manzoor, Saika, Parvez, Tabasum, Ahmad, Dar Nazir, Rashid, Fouzia
• Format: Article
• Language: English
• Subjects: Atherosclerosis; Hyperandrogenism; Insulin resistance; Paraoxonase1; Polycystic ovary syndrome
• ISSN: 2214-5400; 2214-5400
• DOI: 10.1016/j.mgene.2019.100552

Polycystic ovary syndrome (PCOS) is considered as one of the most common endocrine disorders in premenopausal women with varied pathophysiology. In this study we investigated the association between -108C/T and 55L/M polymorphisms of Paraoxonase1 (PON1) gene with PCOS. PON1 gene codes for the antioxidant enzyme PON1 which is an HDL associated enzyme that prevents oxidative modification of low-density lipoprotein. A total of 192 women with PCOS and 212 control women from an ethnic population (Kashmir) were included in the study. The PON1 polymorphism was genotyped using PCR-RFLP technique. Clinical, Biochemical parameters were analyzed, and HOMA-IR was used to estimate insulin resistance. Serum PON1 activity and MDA levels were also measured. The frequencies of variant PON1 -108TT genotype and T allele were significantly higher in women with PCOS than controls (31.25%Vs15.09%) and (56.77%Vs41.27%) respectively. The -108TT genotype remained a significant predictor for PCOS (OR-odds ratio = 3.8051, 95%CI = 2.101–6.8914, χ2-chi square = 20.19, P