Polynucleotide phosphorylase (PNPase) is required for Salmonella enterica serovar Typhimurium colonization in swine

The pnp gene encodes polynucleotide phosphorylase, an exoribonuclease involved in RNA processing and degradation. A mutation in the pnp gene was previously identified by our group in a signature-tagged mutagenesis screen designed to search for Salmonella enterica serovar Typhimurium genes required f...

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Bibliographic Details
Published in:Microbial pathogenesis 2013-12, Vol.65, p.63-66
Main Authors: Bearson, S.M.D., Bearson, B.L., Lee, I.S., Kich, J.D.
Format: Article
Language:English
Subjects:
Animals
cecum
Colonization
Feces - microbiology
fever
Gastrointestinal Tract - microbiology
genes
ileum
lymph nodes
mutagenesis
mutants
Mutation
PNPase
Polynucleotide phosphorylase
Polyribonucleotide Nucleotidyltransferase - genetics
Polyribonucleotide Nucleotidyltransferase - metabolism
RNA
Salmonella
Salmonella Infections, Animal - enzymology
Salmonella Infections, Animal - genetics
Salmonella Infections, Animal - microbiology
Salmonella Typhimurium
Salmonella typhimurium - enzymology
Salmonella typhimurium - genetics
stomach
Swine
Swine - microbiology
Swine Diseases - enzymology
Swine Diseases - genetics
Swine Diseases - microbiology
tonsils
Virulence
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Summary:The pnp gene encodes polynucleotide phosphorylase, an exoribonuclease involved in RNA processing and degradation. A mutation in the pnp gene was previously identified by our group in a signature-tagged mutagenesis screen designed to search for Salmonella enterica serovar Typhimurium genes required for survival in an ex vivo swine stomach content assay. In the current study, attenuation and colonization potential of a S. Typhimurium pnp mutant in the porcine host was evaluated. Following intranasal inoculation with 109 cfu of either the wild-type S. Typhimurium χ4232 strain or an isogenic derivative lacking the pnp gene (n = 5/group), a significant increase (p 
ISSN:0882-4010
1096-1208
DOI:10.1016/j.micpath.2013.10.001