In vitro assessment of Argemone mexicana, Taraxacum officinale, Ruta chalepensis and Tagetes filifolia against Haemonchus contortus nematode eggs and infective (L3) larvae

Argemone mexicana, Taraxacum officinale, Ruta chalepensis and Tagetes filifolia are plants with deworming potential. The purpose of this study was to evaluate methanolic extracts of aerial parts of these plants against Haemonchus contortus eggs and infective larvae (L3) and identify compounds respon...

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Bibliographic Details
Published in:Microbial pathogenesis 2017-08, Vol.109, p.162-168
Main Authors: Jasso Díaz, Gabriela, Hernández, Glafiro Torres, Zamilpa, Alejandro, Becerril Pérez, Carlos Miguel, Ramírez Bribiesca, J. Efrén, Hernández Mendo, Omar, Sánchez Arroyo, Hussein, González Cortazar, Manasés, Mendoza de Gives, Pedro
Format: Article
Language:English
Subjects:
Animals
Anthelmintics - pharmacology
Argemone - chemistry
Chlorogenic acid
Chlorogenic Acid - analogs & derivatives
Chlorogenic Acid - pharmacology
Eggs
Face - parasitology
Haemonchus - drug effects
Haemonchus contortus
Larva - drug effects
Lethal Dose 50
Mexico
Nematode Infections
Plant extracts
Plant Extracts - chemistry
Plant Extracts - pharmacology
Ruta - chemistry
Secondary Metabolism
Secondary metabolites
Sheep
Sheep Diseases - drug therapy
Sheep Diseases - parasitology
Tagetes - chemistry
Taraxacum - chemistry
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Summary:Argemone mexicana, Taraxacum officinale, Ruta chalepensis and Tagetes filifolia are plants with deworming potential. The purpose of this study was to evaluate methanolic extracts of aerial parts of these plants against Haemonchus contortus eggs and infective larvae (L3) and identify compounds responsible for the anthelmintic activity. In vitro probes were performed to identify the anthelmintic activity of plant extracts: egg hatching inhibition (EHI) and larvae mortality. Open column Chromatography was used to bio-guided fractionation of the extract, which shows the best anthelmintic effect. The lethal concentration to inhibit 50% of H. contortus egg hatching or larvae mortality (LC50) was calculated using a Probit analysis. Bio-guided procedure led to the recognition of an active fraction (TF11) mainly composed by 1) quercetagitrin, 2) methyl chlorogenate and chlorogenic acid. Quercetagitrin (1) and methyl chlorogenate (2) did not show an important EHI activity (3–14%) (p 
ISSN:0882-4010
1096-1208
DOI:10.1016/j.micpath.2017.05.048