Determination of benzhexol hydrochloride by capillary zone electrophoresis with an end-column electrochemiluminescence detection

A capillary zone electrophoresis with end-column electrochemiluminescence (ECL) detector was described for the determination of benzhexol hydrochloride. The detection was based on the tris(2,2′-bypyridine)ruthenium(II) [Ru(bpy) 3 2+] ECL reaction with the analyte. Electrophoresis was performed using...

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Bibliographic Details
Published in:Microchemical journal 2004-02, Vol.76 (1), p.11-16
Main Authors: Yan, Jilin, Liu, Jifeng, Cao, Weidong, Sun, Xiuhua, Yang, Xiurong, Wang, Erkang
Format: Article
Language:English
Subjects:
Benzhexol hydrochloride
Capillary electrophoresis
Electrochemiluminescence
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Summary:A capillary zone electrophoresis with end-column electrochemiluminescence (ECL) detector was described for the determination of benzhexol hydrochloride. The detection was based on the tris(2,2′-bypyridine)ruthenium(II) [Ru(bpy) 3 2+] ECL reaction with the analyte. Electrophoresis was performed using a 25 μm i.d. uncoated capillary. 10 mM sodium phosphate buffer (pH=8.0) was used as the running buffer. The solution in the detection cell was 80 mM sodium phosphate (pH=8.0) and 5 mM Ru(bpy) 3 2+. A linear calibration curve of three-orders of magnitude was obtained (with a correlation coefficient of >0.999) from 1.0×10 −8 to 1.0×10 −5 M and the limit of detection was 6.7×10 −9 M ( S/ N=3). This just provides an easy and sensitive method to determine the active ingredient in pharmaceutical formulations.
ISSN:0026-265X
1095-9149
DOI:10.1016/j.microc.2003.10.003