Immunosensor assembled on polymeric nanostructures for clinical diagnosis of C-reactive protein

• Published in: Microchemical journal 2017-07, Vol.133, p.572-576
• Main Authors: Lemos, Anderson J.G., Balvedi, Renata P.A., Rodovalho, Vinicius R., Resende, Laise O., Castro, Ana C.H., Cuadros-Orellana, Sara, Madurro, João M., Brito-Madurro, Ana G.
• Format: Article
• Language: English
• Subjects: Antigen-antibody recognition; Biosensors; C-reactive protein; Cardiovascular diseases; Poly(3-aminothiophenol)
• ISSN: 0026-265X; 1095-9149
• DOI: 10.1016/j.microc.2017.04.026

This paper describes the development of an immunosensor for the c-reactive protein (CRP), based on both direct and indirect electrochemical detections. The anti-CRP probe was grafted to the platform sensitized with poly(3-aminothiophenol) and the interaction of the CRP target with the immunosensor was carried either directly or indirectly, using 4-aminoantipyrine (4-APP) as indicator. The immunosensor presents a linear response in the range from 75ng·mL−1 to 150μg·mL−1 and detection limit of 7.24μg·mL−1 (N=3). The biosensor specificity, which was determined in the presence of non-specific target is also presented in this paper. Assays realized conducted with real samples indicate that the immunosensor discriminates the serum samples from healthy and sick patients with efficiency. In addition, through atomic force microscopy, it was possible to observe differences between the immunosensor surface in the absence or presence of the CRP target. •Specific CRP detection based on poly-3-aminothiophenol matrix.•Electrochemical detection of the protein specific using aminoantypirine as indicator.•The detection limit was 75ng.mL-1, with a correlation coefficient of 0.997 (n=3).•The system discriminates negative serum and positive serum.