An automated homogeneous liquid-liquid microextraction based on deep eutectic solvent for the HPLC-UV determination of caffeine in beverages

• Published in: Microchemical journal 2019-01, Vol.144, p.469-473
• Main Authors: Shishov, Andrey, Volodina, Natalia, Nechaeva, Daria, Gagarinova, Svetlana, Bulatov, Andrey
• Format: Article
• Language: English
• Subjects: Automation; Caffeine; Deep eutectic solvent; Flow analysis; Homogeneous liquid-liquid microextraction; HPLC-UV; Soft drinks
• ISSN: 0026-265X; 1095-9149
• DOI: 10.1016/j.microc.2018.10.014

This work presents an automated homogeneous liquid-liquid microextraction procedure for separation of caffeine from beverages. The microextraction procedure involved mixing sample and water-miscible deep eutectic solvent (DES, choline chloride with phenol at a 1:3 molar ratio) into a mixing chamber of a flow system resulting formation of homogeneous solution. Injection of aprotic solvent (tetrahydrofuran) into homogeneous sample solution promoted the fine DES droplets formation and caffeine extraction into DES phase separated. The automated homogeneous liquid-liquid microextraction procedure was coupled with a HPLC-UV system. The conditions of caffeine microextraction and its HPLC-UV determination in DES phase were studied and optimized. Under optimal experimental conditions the linear detection range and limit of detection, calculated from a blank test, based on 3σ were found to be 0.1–200 mg L−1 and 0.03 mg L−1, respectively. The application of the developed procedure was demonstrated in the determination of caffeine in commercial soft drinks samples. •Automated homogeneous liquid-liquid microextraction (A-HLLME) using deep eutectic solvent (DES)•DES based on choline chloride with phenol as an extractant for caffeine extraction•A-HLLME coupled with HPLC-UV•Automated method for the determination of caffeine in beverages