Advance methods for the qualitative and quantitative determination of microorganisms

• Published in: Microchemical journal 2021-07, Vol.166, p.106188, Article 106188
• Main Authors: Ayhan, Kamuran, Coşansu, Serap, Orhan-Yanıkan, Esin, Gülseren, Gülcihan
• Format: Article
• Language: English
• Subjects: Biosensor; Chemical characterization; Identification; Microorganisms; Molecular techniques; SERS
• ISSN: 0026-265X; 1095-9149
• DOI: 10.1016/j.microc.2021.106188

•Advances of new molecular techniques are discussed from various aspects.•Next generation methods are addressed for microbial identification.•The chemical analysis methods proposed for identification of microorganism.•Overview of different techniques provided basis for sample based method selection.•Biosensors can detect microorganisms with high sensitivity and selectivity.•Biosensors are promising tools for clinical, environmental and food microbiology. The next generation microbiological analysis is progressed swiftly due to its numerous advantages such as sensitivity, reproducibility, accuracy, low cost and rapidity. Although, conventional culture and analytical methods frequently used for the microbiological examination in terms of its easily accessible, advanced methods provide improving its selectivity and sensitivity. The present paper reviews a variety of qualitative and quantitative techniques for determination of both commonly and rarely emerged microorganisms such as Escherichia coli, Staphylococcus aureus, Listeria monocytogenes, Acinetobacter, Rothia, Pantoea, Cryptococcus, Bacillus, Micrococcus etc. that isolated from different type of sources. Presented analytic, nucleic acid-based, biosensor-based, bacteriophage-based and spectroscopy-based methods are detailed with respect to their fundamental principles and examples of applications in microbial ecology. Future-oriented evaluations of these methods and their applications are also discussed to indicate their significance in the field.