Development and optimization of a method based on QuEChERS-dSPE followed by UPLC-MS/MS for the simultaneous determination of 21 mycotoxins in nutmeg and related products

•QuEChERS-dSPE-UPLC-MS/MS method was developed for 21 mycotoxins.•Quantification was made by matrix-matched combined with internal standard.•Validation data were consistent with European Commission guideline requirements.•Aflatoxins were detected in nutmeg.•The proposed method is effective for the d...

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Bibliographic Details
Published in:Microchemical journal 2021-09, Vol.168, p.106499, Article 106499
Main Authors: Zhao, Xiangsheng, Liu, Dan, Zhang, Lei, Zhou, Yakui, Yang, Meihua
Format: Article
Language:English
Subjects:
Aflatoxins
Mycotoxins
Myristica fragrans
UPLC-MS/MS
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Summary:•QuEChERS-dSPE-UPLC-MS/MS method was developed for 21 mycotoxins.•Quantification was made by matrix-matched combined with internal standard.•Validation data were consistent with European Commission guideline requirements.•Aflatoxins were detected in nutmeg.•The proposed method is effective for the determination of multi-mycotoxins. Nutmeg (Myristica fragrans), a popular spice and traditional medicine worldwide, is susceptible to fungal and mycotoxin contamination. Hence, it is important to monitor the residual levels of multiple mycotoxins in nutmeg and related products. However, analysts are continuously faced with the challenges of quantifying mycotoxin concentrations in complex matrices such as spices and traditional Chinese medicines. To develop a simple and sensitive mycotoxin analysis method encompassing the total workflow from sample preparation to quantitative analysis, a simple, robust, and sensitive method for the simultaneous identification and quantification of 21 mycotoxins in nutmeg and related products was described. The method is based on sample preparation by modified QuEChERS extraction and dispersive solid-phase extraction (dSPE) cleanup followed by ultrahigh-performance liquid chromatography-triple quadrupole tandem mass spectrometry (UPLC-MS/MS). Matrix-matched calibration was applied for the quantification of the investigated mycotoxins. The limits of detection of the target analytes ranged from 0.04 μg/kg to 6.0 μg/kg. Recovery rates ranged from 60.49% to 97.46% at three spiking levels, with relative standard deviations
ISSN:0026-265X
1095-9149
DOI:10.1016/j.microc.2021.106499