Development of a gold nanoparticle-based lateral flow immunoassay for the detection of pyridaben

• Published in: Microchemical journal 2021-11, Vol.170, p.106762, Article 106762
• Main Authors: Liu, Jie, Xu, Xinxin, Wu, Aihong, Wang, Zhengyou, Song, Shanshan, Kuang, Hua, Liu, Liqiang, Xu, Chuanlai
• Format: Article
• Language: English
• Subjects: Apple; Cucumber; Immunochromatographic assay; Monoclonal antibody; Pyridaben
• ISSN: 0026-265X; 1095-9149
• DOI: 10.1016/j.microc.2021.106762

[Display omitted] •A novel hapten of pyridaben was designed.•The mAb had a 50% inhibitory concentration (IC50) of 2.36 ng/mL.•The LFIA had cut-off values of 25 ng/g and 50 ng/g for pyridaben in cucumber and apple samples, respectively.•The LFIA was comparable to ic-ELISA and LC-MS. In this study, a novel hapten of pyridaben was designed and used in the preparation of a monoclonal antibody (mAb), which was then used in the development of a lateral flow immunoassay (LFIA). The mAb 3C2 had good sensitivity with a 50% inhibitory concentration (IC50) of 2.36 ng/mL. The developed LFIA had good sensitivity with cut-off values of 25 and 50 ng/g for pyridaben in cucumber and apple samples, respectively. Importantly, our LFIA was comparable to other methods of detection such as indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) and liquid chromatography mass spectrometry (LC-MS/MS). These results indicated that the LFIA method represents a reliable, portable, and high-throughput method for the on-site detection of pyridaben in vegetables and fruits.