Simultaneous determination of fungicides and carbamates in tobacco by ultra performance convergence chromatography-tandem mass spectrometry coupled with modified QuEChERS

• Published in: Microchemical journal 2021-12, Vol.171, p.106849, Article 106849
• Main Authors: Yang, Fei, Tang, Gangling, Ye, Changwen, Wang, Ying, Fan, Meijuan, Deng, Huimin, Liu, Shanshan, Bian, Zhaoyang, Ji, Yuan
• Format: Article
• Language: English
• Subjects: Carbamates; Fungicides; Graphene; Modified QuEChERS; Tobacco; UPC2-MS/MS
• ISSN: 0026-265X; 1095-9149
• DOI: 10.1016/j.microc.2021.106849

[Display omitted] •We developed a modified QuEChERS coupled SFC-MS/MS method for the simultaneous determination of fungicide and carbamate pesticides in tobacco.•Graphene was applied to DSPE for the determination of fungicide and carbamate pesticides in tobacco.•This method possessed the advantages of short separation time and low consumption of organic solvents.•The proposed method is a fast, simple and green method. This study concerned the development and validation of a green and rapid method for the analysis of 23 pesticides in tobacco using ultra performance convergence chromatography-tandem mass spectrometry (UPC2-MS/MS) combined with modified QuEChERS. The sample was extracted with acetonitrile, purified by graphene, and then determined by UPC2-MS/MS. In this paper, optimization was made on factors affecting separation efficiency including chromatographic column, modifier, column temperature, and back pressure. With CO2-ethanol as mobile phase, all pesticides were adequately separated on a Viridis HSS C18 SB column, and the whole analysis process was completed within 6 min. Under optimal conditions, the calibration curves showed good linearity in the range of 10–200 ng/mL with the regression coefficients higher than 0.9946. The limit of detection and limit of quantification were 1.04–7.07 and 3.32–23.16 μg/kg, respectively. At three spiked levels (0.2, 1.0 and 2.0 mg/kg), mean recoveries for 23 pesticides were ranging from 80.2% to 110.6% and relative standard deviations were 2.2%–7.2%. The proposed method, being fast, sensitive, and practical, can be used for rapid detection of fungicides and carbamates in tobacco.