Novel biocompatible amide phthalocyanine for simultaneous electrochemical detection of adenine and guanine

• Published in: Microchemical journal 2022-04, Vol.175, p.107223, Article 107223
• Main Authors: Shantharaja, Nemakal, Manjunatha, Giddaerappa, Gopal Hegde, Subramanya, Koodlur Sannegowda, Lokesh
• Format: Article
• Language: English
• Subjects: Adenine (A); Amide Phthalocyanine; Amperometry; Guanine (G); Sensing
• ISSN: 0026-265X
• DOI: 10.1016/j.microc.2022.107223

[Display omitted] •Novel biocompatible amide bridged copper phthalocyanine (CuTPTAPc) was prepared and structure was elucidated using physicochemical techniques.•Immobilised CuTPTAPc/GCE was characterised and employed for simultaneous detection of guanine (G) and adenine (A) nucleic bases.•CuTPTAPc/GCE displayed better linear range, sensitivity and selectivity for (G) and (A) by DPV and chronoamperometric techniques.•Designed electrode was stable and can be applied for real sample analysis. Biomolecules like adenine and guanine are important nucleic bases present in DNA and RNA molecules and have significant role in the human body for healthy growth and development. Their alteration and deficiency in their concentration lead to malfunction and abnormal growth and hence, these molecules need to be monitored continuously. Here, copper tetracarboxylic acid phthalocyanine (CuIITCAPc) is synthesized and converted to novel amide linkage 4-(pyridine-3-yl)-4,5-dihydro-1,3-thiazol- copper phthalocyanine (CuIITPTAPc). Synthesized macrocycles are characterized by various physico-analytical techniques which inferred that the synthesized complexes are pure. CuIITPTAPc film was administered on glassy carbon electrode (GCE) and used for the simultaneous determination and monitoring of biologically important adenine (A) and guanine (G) bio-molecules. The GCE/CuIITPTAPc showed significantly better voltammetric response for the simultaneous detection of guanine and adenine in the quantitative range 5 to 35 μM. Differential pulse voltammetry (DPV) using CuIITPTAPc electrode exhibited linear response in the quantitative range 5 to 70 µM with limit of detection (LOD) 1.42 μM for simultaneous detection of guanine and adenine. The chronoamperometric (CA) method displayed linear response in the range 2 to18 and 3 to 27 µM with LOD of 0.66 μM and 1.00 μM for G and A, respectively. The molecules which co-exist with G and A did not respond during CA analysis inferring superior selectivity of the proposed sensor for nucleic acid bases. The designed electrode satisfactorily employed for the real sample analysis.