Silver nanosol RRS aptamer assay of trace glyphosate based on gold-doped polystyrene nanocatalytic amplification

• Published in: Microchemical journal 2022-05, Vol.176, p.107252, Article 107252
• Main Authors: Wang, Zhenghong, Shu, Yiyi, Li, Jingjing, Liang, Aihui, Jiang, Zhiliang
• Format: Article
• Language: English
• Subjects: Apt RRS method; Gly; Gold-doped polystyrene nanospheres; Nanocatalytic amplification
• ISSN: 0026-265X; 1095-9149
• DOI: 10.1016/j.microc.2022.107252

A nanosilver RRS aptamer assay was proposed for trace glyphosate based on gold-doped polystyrene nanocatalytic amplification. [Display omitted] •The nanoreaction of Ag+-formate was used as new indicator.•Gold-doped polystyrene nanoenzyme strong catalyzed the nanoreaction.•The nanocatalytic amplification was coupled with glyphosate ampater.•The RRS assay was sensitive and selective. In this paper, carbon monoxide is used as a reducing agent to establish a new procedure for preparing gold-doped polystyrene nanoenzyme (Au@PN) sol, that was characterized by electron microscopy, molecular spectroscopy and other techniques. It can effectively catalyze the AgNO3-sodium formate (SF) reaction to generate silver nanoparticles with a significant resonance Rayleigh scattering (RRS) signal. A new RRS assay for detecting trace amounts of glyphosate (Gly) in soil was developed by combining the nanocatalytic amplification signal and the specific aptamer (Apt) reaction. The linear range is 0.5–20 nM, and the detection limit is 0.24 nM. On the one hand, it enriched the research of polystyrene nanoenzymes and expanded the application of polystyrene nanospheres. On the other hand, an Apt RRS assay platform for detecting other target molecules was constructed.